Analysis Note

ControlMouse brain tissue lysate

Application

Anti-Aldh1L1, clone N103/39 is an antibody targeting the Aldh1L1 protein, validated for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

Research CategoryNeuroscience

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Aldh1L1 in 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Aldh1L1 rat cerebral cortex tissue and human pons/midbrain tissue.

Immunofluorescence Analysis: A representative lot detected Aldh1L1 in rat cortex and cerebellum tissue.

Research Sub CategorySensory & PNS

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Aldh1L1, also known as 10-formyltetrahydrofolate dehydrogenase (10-FTHFDH), is a multidomain protein that is found in liver cytosol and also serves as a CNS astrocyte marker. Aldh1L1 is comprised of two functionally unrelated domains, an aldehyde dehydrogenase-homologous domain and a folate-binding hydrolase domain. These two domains are thought to be connected by a 100-residue linker. It is known to induce phosphorylation of p53 at Ser6, which is a critical step in stimulating apoptosis.

Immunogen

Recombinant full-length rat Aldh1L1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Aldh1L1 in 10 µg of mouse brain tissue lysate.

Specificity

This Aldh1L1 monoclonal antibody does not exhibit cross-reactivity toward Aldh1L2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~99 kDa observed