Application

Research CategoryNeuroscience

Anti-FMR1, clone 1F1, Cat. No. MABN2453, is a highly specific mouse monoclonal antibody that targets Fragile X mental retardation protein 1 homolog and has been tested for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Synaptic functional regulator FMR1 (UniProt: P35922; also known as Fragile X mental retardation protein 1 homolog, FMRP, Protein FMR-1, mFmr1p) is encoded by the Fmr1 (also known as Fmr-1) gene (Gene ID: 14265) in murine species. FMR-1 is a homodimeric multifunctional polyribosome-associated RNA-binding protein predominantly found in the brain. It can also form heterodimer with FXR1 in a methylation-dependent manner. It shuttles between nucleus and cytoplasm in a XPO1/CRM1-dependent manner and plays a central role in neuronal development and synaptic plasticity through the regulation of alternative mRNA splicing, mRNA stability, mRNA dendritic transport and postsynaptic local protein synthesis of a subset of mRNAs. FMR-1 plays a role in mRNA nuclear export and together with export factor NXF2, it is involved in the regulation of the NXF1 mRNA stability in neurons. It stabilizes the scaffolding postsynaptic density protein DLG4/PSD-95 and the myelin basic protein mRNAs in hippocampal neurons and glial cells, respectively and this stabilization is further increased in response to metabotropic glutamate receptor (mGluR) stimulation. In response to mGluR stimulation, FMR1-target mRNAs are rapidly derepressed, allowing for local translation at synapses. FMR-1 is shown to play a role in the modulation of the sodium-activated potassium channel KCNT1 gating activity and negatively regulate the voltage-dependent calcium channel current density in soma and presynaptic terminals of dorsal root ganglion (DRG) neurons. FMR-1 is phosphorylated on several serine residues and phosphorylation at serine 499 is required for phosphorylation of other nearby serine residues. Although phosphorylation has no effect on the binding of individual mRNA species, it may affect the association with polyribosome.

Immunogen

Full-length recombinant mouse Fragile X mental retardation protein 1 homolog (FMR1).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected FMR1 in HeLa cell lysate.

Specificity

Clone 1F1 is a mouse monoclonal antibody that specifically detects Fragile X mental retardation protein 1 homolog (FMR1). It targets an epitope with in the C-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~72 kDa observed; 68.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).