Analysis Note

ControlT47D cell lysate

Application

This Anti-ROR gamma T Antibody, clone 6F3.1 is validated for use in WB, IH for the detection of ROR gamma T.

Research CategoryInflammation & Immunology

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected ROR gamma T in normal human liver tissues.

Research Sub CategoryInflammation & Autoimmune Mechanisms

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Retinoid-related orphan receptor-gamma t (RORγT) is one of two RORγ isoforms belonging to the nuclear hormone receptor family and contains a single nuclear receptor DNA-binding domain. RORγT is a negative regulator of TNFDF6/FASL expression and T-cell production of IL2. The upregulation of RORγT is a requirement for TH17 cell differentiation. Inhibition of RORγT is induced by TGF-β's interaction with Foxp3. Expression is observed mainly in immature thymocytes, and, during fetal development, is found exclusively in lymphoid-inducer (LTi) cells.

Immunogen

GST-tagged recombinant protein corresponding to human ROR gamma T.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluted in Western Blot in T47D cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected ROR gamma T in 10 µg of T47D cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~55 kDa observed. Two isoforms at ~55 kDa and ~58 kDa may be observed in some cell lysates.