Anti-Thy-1; clone M5/49

Analysis Note

ControlP388D1 cell lysate

Application

Research Sub CategoryInflammation & Autoimmune MechanismsNeural Stem Cells

Functional Assay: A previous lot of this antibody was used for the inhibition of B6 Anti-BNLφ CTL mediated cell death. (Davignon, D., et al. (1981). PNAS USA. 78(7):4535-4539.)

Research CategoryInflammation & Immunology

Anti-Thy-1 Antibody, clone M5/49 detects level of Thy-1 & has been published & validated for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Thy-1 membrane glycoprotein (Thy-1) is a glycosylphosphatidylinositol (GPI)-anchored surface protein belonging to the immunoglobulin superfamily and is a major cell surface protein found on both T–cells and in the brain. Thy-1 is thought to be involved in interactions taking place during synaptogenesis and other neurological events between cells or cells and their ligands. There are two known alleles for Thy-1; Thy1.1 and Thy1.2. Studies have shown N-glycosolation to take place at three locations on the protein.

Immunogen

B6 Anti-BNL4φ lymphocytes

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in P388D1 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Thy-1 in 10 µg of P388D1 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~22 kDa observed.
Higher bands may be observed due to glycosylation.