Analysis Note

ControlNormal human tranverse colon tissue

Application

Western Blot Analysis: 0.5 ug/mL from a previous lot detected alpha defensin-5 (HD5) in human small intestine tissue lysate. The urea-treated method is recommended instead of SDS page (Shen, B. et al., 2005).

Anti-Alpha Defensin-5 (HD5) Antibody, clone 8C8 detects level of Alpha Defensin-5 (HD5) & has been published & validated for use in IH & WB.

General description

Human alpha defensin-5 (HD5) is one of six peptides belonging to the alpha defensin family. Defensins are small peptides involved in immunological defense against viral and microbial infections through either direct interaction with pathogens or in combination with other immune system factors. There are three subfamilies of defensins (α, β, and θ). α defensins are only observed in mammals and are mainly β-sheet structures. Alpha defensin-5 (HD5) is primarily expressed in the Paneth cells found within the small intestine, and has been shown to possess activity against both Gram-positive and Gram-negative microbes. Studies have shown alpha defensin-5 (HD5) to be a strong antagonist towards human Papillomavirus infection. It may also play a role Crohn's disease.

Immunogen

Recombinant protein corresponding to human alpha defensin-5 (HD5).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Evaluated by Immunohistochemistry in normal human tranverse colon tissue.

Immunohistochemistry Analysis: A 1:200 dilution of this antibody detected alpha defensin-5 (HD5) in normal human tranverse colon tissue.

Target description

10 kDa calculated