Application
Anti-IL1RL1 antibody, clone RMST2-2, Azide Free is an antibody against IL1RL1 for use in flow cytometry & activity assay.
Research Sub CategoryImmunoglobulins & Immunology
Activity Assay Analysis: A representative lot from an independent laboratory induces IL-6 production in BMCC cells (Prof. Hisaya Akiba, Jutendo University).
Research CategoryInflammation & Immunology
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
IL1RL1, or Interleukin-1 receptor-like 1 protein, which is also known as Lymphocyte antigen 84, Protein ST2, or Protein T1, and encoded by the gene IL1rL1/Ly84/St2/Ste2 is the receptor for IL-33 and its activation recruits MYD88, IRAK1, IRAK4 and TRAF6 transcription factors and promotes the activation of ERK1/MAPK14 and MAPK8. IL1RL1 also is involved in T-helper Cell activation and function. IL1RL1 is localized to the plasma membrane. IL1RL1 is expressed in hematopoietic tissues, macrophages, epithelial and fibroblast cells lines as well as important tissues such as heart and lung. IL1RL1 is a member of the Toll-like receptor superfamily and interestingly plays an important role not in inflammation but in cardiac protection. When IL-33 binds it elicits a cardioprotective signaling response in the tissue and thus helps protect the heart tissue during times of ischemia for instance. Defects in IL1RL1 have been linked to atopic dermatitis and asthma.
Immunogen
Mouse ST-2/IL1RL1.
Physical form
Protein G Purified
Format: Purified
0.1M Tris Glycine, pH 7.4, 150mM NaCl, without preservatives.
Quality
Evalauted by Flow Cytometry in ST2/L5178Y and L5178Y parent cells.
Flow Cytometry Analysis: 1 µg of this antibody detected IL1RL1 in 1X10E6 ST2/L5178Y cells.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Target description
64 kDa calculated
This product has met the following criteria to qualify for the following awards: