Anti-UBR5; clone 16G10.1

Application

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected UBR5 in human cerebral cortex, human tonsil, and human prostate cancer tissues.

Research CategoryInflammation & Immunology

Anti-UBR5, clone 16G10.1, Cat. No. MABF1110, is a mouse monoclonal antibody that detects E3 ubiquitin-protein ligase UBR5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

E3 ubiquitin-protein ligase UBR5 (UniProt; O95071; also known as EC: 2.3.2.26, E3 ubiquitin-protein ligase, HECT domain-containing 1, HECT-type E3 ubiquitin transferase UBR5, Hyperplastic discs protein homolog, hHYD, Progestin-induced protein) is encoded by the UBR5 (also known as EDD, EDD1, HYD, KIAA0896) gene (Gene ID: 51366) in human. UBR5 is a widely expressed nuclear E3 ligase that is most abundant in testis and expressed at high levels in brain, pituitary and kidney. It is a component of the N-end rule pathway that recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. UBR5 is involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination and may play a role in control of cell cycle progression. It is shown to regulate DNA topoisomerase II binding protein (TopBP1) in the DNA damage response and may have a tumor suppressive function. It acts as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of Lys-63-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes. URB5 contains a zinc-finger domain (aa 1177-1245), a poly(A)-binding protein C-terminal (PABC) domain(aa 2377-2454), and a (Homologous to the E6-AP Carboxyl Terminus (HECT) domain (aa 2462-2799). Two isoforms of UBR5 have been described that are produced by alternative splicing.

Immunogen

GST/His-tagged recombinant fragment corresponding to 181 amino acids from the C-terminal half of human E3 ubiquitin-protein ligase UBR5.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected UBR5 in 10 µg of Raji cell lysate.

Specificity

Clone 16G10.1 detects E3 ubiquitin-protein ligage UBR5 in human cells. It targets an epitope within 181 amino acids from the C-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~305 kDa oserved; 309.35 kDa calculated. Uncharacterized bands may be observed in some lysate(s).