Application

Neutralization Assay Analysis: A representative lot from an independent laboratory detected MIF during the migration of human peripheral blood granulocytes (Rupreht, R. R., et al. (2000). Pflugers Arch. 440(5 Suppl):R78-80.).

This Anti-MIF Antibody, clone M1, preservative free is validated for use in flow cytometry, neutralizing & western blotting for the detection of MIF.

General description

MIF, also known as Macrophage migration inhibitory factor, or Glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, L-dopachrome tautomerase, or Phenylpyruvate tautomerase, and encoded by the gene MIF/GLIF/MMIF, is proinflammatory cytokine involved in the innate immune response to bacterial pathogens. MIF is secreted by macrophages upon stimulation by bacterial lipopolysaccharide or bacterial antigens, and the expression of MIF at sites of inflammation suggests a role as a mediator in regulating the function of macrophages in host defense. MIF also counteracts the anti-inflammatory activity of glucocorticoids and has various enzyme activities but their physiological function is not known. MIF expression is primarily lymphocytes, macrophages and immune system cells. Defects in MIF may be associated with Rheumatoid arthritis systemic juvenile disease, an inflammatory articular disorder with systemic onset beginning at age 16. Also serum levels of MIF are elevated in cases of severe sepsis and are correlated with low survival.

Immunogen

Recombinant protein corresponding to human MIF.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Evaluated by Flow Cytometry by Jurkat cells.

Flow Cytometry: 1 µg of this antibody detected MIF in 1X10E6 Jurkat cells.

Target description

12 kDa calculated