Application

Research Sub CategoryNuclear Receptors

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected SAE2 in human kidney and rat testis tissue.

Anti-SAE2 Antibody, clone 7H8.1 | MABE927 is an antibody against SAE2 Antibody for use in Western Blotting, Immunohistochemistry.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SUMO-activating enzyme subunit 2, also known as Anthracycline-associated resistance ARX, or Ubiquitin-like 1-activating enzyme E1B, and encoded by the gene name UBA2 or SAE2 or UBLE1B, belongs to the ubiquitin-activating E1 family. SUMOs are a family of small, related proteins (SUMO-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. This sumoylation is effected by the heterodimeric enzyme SAE1/SAE2 and the SUMO-1-conjugating enzyme Ubch9. The sumoylation pathway mediated by SAE1/SAE2 is distinct from other ubiquitin-like protein (Ubl) pathways.

Immunogen

GST-tagged recombinant protein corresponding to human SAE2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in Neuro-2a cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SAE2 in 10 µg of Neuro-2a cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~80 kDa observed. The calculated molecular weight is 72 kDa, however SAE2 has been shown as a ~70-90 kDa band in western blots (Truong, K., et al. (2012). J. Biol. Chem. 287(51):42611-42619).