Analysis Note

ControlMouse brain tissue lysate

Application

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected MiTF in human melanoma tissue.

Research Sub CategoryTranscription Factors

Research CategorySignaling

Anti-MiTF Antibody, clone 3D1.2 is an antibody against MiTF for use in Western Blotting & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Microphthalmia-associated transcription factor (MiTF) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, and development of mast cells and bone. Mutations in MiTF cause auditory pigmentary syndromes in humans, such as Waardenburg syndrome type II and type IIa, and Tietz syndrome. There are two known isoforms of MiTF, differing by 66 amino acids at the N-terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, whereas the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Immunogen

GST-tagged recombinant protein corresponding to human MiTF.

Linkage

Replaces: MAB3747

Physical form

Purified by Ion-Exchange Chromatography

Purified mouse monoclonal IgMκ in buffer containing 0.01M PBS, 0.1% sodium azide, pH 7.1.

Format: Purified

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected MiTF in 10 µg of mouse brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~52 kDa observed. Multiple isoforms between 46-56 kDa are known to exist.