Anti-BPTF; clone 15B1.1

Application

Research Sub CategoryNuclear Receptors

This Anti-BPTF Antibody, clone 15B1.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of BPTF .

Research CategoryEpigenetics & Nuclear Function

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected BPTF in human testis, rat colon, and mouse colon tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nucleosome-remodeling factor (NURF) subunit BPTF (UniProt E9Q6A7 & A2A654; also known as Bromodomain and PHD finger-containing transcription factor, Fetal Alzheimer antigen) is encoded by the Bptf (also known as 9430093H17Rik, Falz) gene (Gene ID 207165) in murine species. ATP-dependent chromatin remodeling complexes are classified into four major subfamilies (SWI/SNF, ISWI, CHD, and INO80). NURF is the founding member of ISWI remodeling complexes. Mammalian NURF is composed of BPTF, the ATPase SNF2L, and the Trp-Asp (WD) repeat protein pRBAP46/48, with BPTF being the largest subunit among the three. BPTF PHD finger-mediated binding of H3K4me3 stabilizes the NURF complex on chromatin, resulting in NURF-catalyzed nucleosome sliding. The NURF remodeling complex is essential for mammalian development and executes epigenetic regulation in multiple signaling pathways, including heat shock, TGF-beta/SMAD, JAK/STAT, WNT/beta-catenin, RB, and nuclear hormone receptors.

Immunogen

GST-tagged recombinant protein corresponding to mouse BPTF.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected BPTF in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~322 kDa observed. Uncharacterized band(s) may appear in some lysates.