Anti-phospho Histone H2B (Tyr37)clone 15

Application

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated phospho Histone H2B (Tyr37) in mouse MEF cells which were untreated or treated with WEE inhibitor (Mahajan, K., et al. (2012). Nat Struct Mol Biol. 19(9):930-937.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated phospho Histone H2B (Tyr37) in MEF-overexpressing WEE (Mahajan, K., et al. (2012). Nat Struct Mol Biol. 19(9):930-937.).

This Anti-phospho Histone H2B (Tyr37) antibody, clone 15 is validated for use in western blotting & ChIP for the detection of phospho Histone H2B (Tyr37).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Immunogen

KLH-conjugated linear peptide corresponding to human Histone H2B phosphorylated at Tyr37.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H2B (Tyr37) in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~17 kDa observed