Anti-Sirt1(Sir2); clone 3-10; Rb Mono

Application

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Sirt1(Sir2) in mouse cerebral cortex tissue sections.

Anti-Sirt1(Sir2), clone 3-10, Cat. No. MABE426-I, is a highly specific rabbit monoclonal antibody that targets NAD-dependent protein deacetylase sirtuin-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NAD-dependent protein deacetylase sirtuin-1 (UniProt: Q923E4; also known as Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SIR2alpha, Sir2, mSIR2a, Sirt-1) is encoded by the Sirt1 (also known as Sir2l1) gene (Gene ID: 93759) in murine species. Sirt1 is a NAD-dependent protein deacetylase that is widely expressed and links transcriptional regulation directly to intracellular energetics. In liver, Sirt1 is expressed in a circadian manner with maximal and minimal levels reaching at around Zeitgeber time (ZT) 16 and ZT4, respectively and its deacetylase activity is also regulated in a circadian manner that peaks at ZT15. It participates in the coordination of several cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis, and autophagy. It is shown to modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. It deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression in both positive and negative manner. Sirt1 serves as a sensor of the cytosolic ratio of NAD+/NADH, which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Sirt1 has four nucleotide (NAD)-binding regions and four zinc binding sites. It is reported to shuttle between nucleus and cytoplasm. Sir1 is proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield a catalytic inactive but stable 75 kDa fragment (aa 2-525). Sirt1 can be phosphorylated by multiple protein kinases. It is phosphorylated by STK4/MST1 that results in inhibition of SIRT1-mediated p53/TP53 deacetylation and phosphorylation by MAPK8/JNK1 at serine 46 and threonine 522 is reported to increase its nuclear localization and enzymatic activity. Sirt1 phosphorylation at threonine 522 by DYRK1A and DYRK3 activates its deacetylase activity and can promote cell survival.

Immunogen

His-tagged full-length recombinant mouse NAD-dependent protein deacetylase sirtuin-1 (Sirt1).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit monoclonal antibody in cell culture supernatant with 0.05% sodium azide.

Format: Unpurified

Unpurified

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Sirt1(Sir2) in NIH/3T3 cell lysate.

Specificity

Clone 3-10 specifically detects murine Sirt1.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~110 kDa observed; 80.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).