Application

Research Sub CategoryApoptosis - Additional

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MDM2 in A431 cells.
The unconjugated antibody (Cat. Nos. MABE340 & OP46) is shown to be suitable also for immunohistochemistry and Western blotting applications.

This Anti-MDM2 Antibody, clone IF2, Alexa Fluor^® 488 Conjugate is validated for use in Immunocytochemistry for the detection of MDM2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

E3 ubiquitin-protein ligase Mdm2 (UniProt Q00987; also known as Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2) is encoded by the MDM2 gene (Gene ID 4193) in human. MDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes p53 transcriptional activity by binding to and blocking p53 N-terminal trans-activation domain. MDM2 can also monoubiquitinate p53, thereby promoting p53 cytoplasmic localization to prevent p53 nuclear funtion. In addition, MDM2, when in complex with p300, also mediates p53 polyubiquitination, resulting in p53 proteosomal degradation. MDM2 overexpression is shown to inhibit DNA double-strand break repair mediated through its interaction with Nbs1 in a p53-independent manner.

Immunogen

Recombinant human MDM2.

Epitope: Within amino acids 26-169.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG2b antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Protein G purified.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MDM2 in HeLa cells.

Specificity

Clone IF2 recognizes the ~85/90 kDa (apparent MW) MDM2, as well as isoforms of ~57/60 kDa and ~74/76 kDa by immunoblotting using the unconjugated antibody (Cat. Nos. MABE340 & OP46).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

~85 kDa observed.