Anti-JARID1D/KDM5D; clone 7F10.4

Application

This Anti-JARID1D Antibody, clone 7F10.4 is validated for use in Western Blotting and Immunocytochemistry for the detection of KDM5D .

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected JARID1D/KDM5D in HeLa cells.

General description

Lysine-specific demethylase 5D (UniProt Q9BY66; also known as H-Y, Histone demethylase JARID1D, Histocompatibility Y antigen, Human homolog of selected mouse cDNA on Y, Jumonji/ARID domain-containing protein 1D, Jumonji AT rich interactive domain 1D (RBP2-like), Protein SmcY, SMC homolog Y chromosome, Smcy homolog Y-linked) is encoded by the KDM5D (also known as HY, HYA, JARID1D, KIAA0234, SMCY) gene (Gene ID 8284) in human. JARID1D/KDM5D is a histone demethylase that specifically catalyzes the demethylation of tri- and di-methylated Lys4 of histone H3 (H3K4m3 and H3K4m2), but not methylated H3K9, H3K27, H3K36, H3K79, H4K20, or monomethylated H3K4. The KDM5D gene is mapped to the azoospermia factor (AZF) region known to be involved in spermatogenesis.

Immunogen

KLH-conjugated linear peptide corresponding to human JARID1D/KDM5D.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected JARID1D/KDM5D in 10 µg of U2OS cell lysate.

Specificity

Clone 7F10.4 is expected to react with all three spliced JARID1D/KDM5D isoforms.

Target description

~200 kDa observed. Isoform 1 (174.1 kDa calculated); Isoform 2 (167.4 kDa calculated); Isoform 3 (177.6 kDa calculated). Uncharacterized band(s) may appear in some lysates.