Application

Research CategoryEpigenetics & Nuclear Function

Anti-ZP-2, clone IE-3, Cat. No. MABE1142, is a highly specific rat monoclonal antibody that targets Zona pellucida sperm-binding protein 2 and has been tested in ELISA, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Immunoprecipitation Analysis: A representative lot detected ZP-2 in Immunoprecipitation applications (East, I.J., et. al. (1984). J Cell Biol. 98(3):795-800).

Immunofluorescence Analysis: A representative lot detected ZP-2 in Immunofluorescence applications (East, I.J., et. al. (1984). J Cell Biol. 98(3):795-800).

Immunofluorescence A representative lot detected ZP-2 in Immunofluorescence applications (Avella, M.A., et. al. (2016). Sci Transl Med. 8(336):336ra60).

ELISA Analysis: A representative lot detected ZP-2 in Western Blotting applications (East, I.J., et. al. (1984). J Cell Biol. 98(3):795-800; Sun, W., et. al. (1999). Biol Reprod. 60(4):900-7).

Western Blotting Analysis: A representative lot detected ZP-2 in ELISA applications (Sun, W., et. al. (1999). Biol Reprod. 60(4):900-7).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Zona pellucida sperm-binding protein 2 (UniProt: P20239; also known as Zona pellucida glycoprotein 2, Zp-2, Zona pellucida protein A) is encoded by the Zp2 (also known as Zp-2, Zpa) gene (Gene ID: 22787) in murine species. The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The mammalian zona pellucida mediates species-specific sperm binding, induction of the acrosome reaction, and prevents post-fertilization polyspermy. ZP2 is expressed during the 2-week growth phase of oogenesis, prior to ovulation and may act as a secondary sperm receptor and is proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL and is exocytosed from cortical granules after fertilization, yielding an N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage plays an important role in the post-fertilization block to polyspermy. Female mice lacking Zp2 gene do not form a stable zona matrix and are sterile. (Ref.: Hinsch, KD and Hinsch, E. (1999). Andrologia.31(5):320-322.)

Immunogen

Epitope: N-terminus

Purified mouse zonae pellucidae.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G purified

Format: Purified

Quality

Evaluated by Western Blotting in mouse ovary tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ZP-2 in 10 µg of mouse ovary tissue lysate.

Specificity

Clone IE-3 detects Zona pellucida sperm-binding protein 2 in murine species.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~120 kDa observed; 80.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).