Application

Anti-SALL2, clone 2B9.1, Cat. No. MABD633, is a highly specific mouse monoclonal antibody that targets Sal-like protein 2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research CategoryNeuroscience

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected SALL2 in human cerebellum tissues.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Sal-like protein 2 (UniProt: Q9Y467; also known as Zinc finger protein 795, Zinc finger protein SALL2, Zinc finger protein Spalt-2, Sal-2, hSal2) is encoded by the SALL2 (also known as KIAA0360, SAL2, ZNF795) gene (Gene ID: 6297) in human. SALL2 is a member of the Sal C2H2-type zinc-finger protein family that serves as a transcription factor and plays a role in eye development before, during, and after optic fissure closure. Two isoforms of SALL2 have been described (105 kDa and 21 kDa) that are produced by alternative splicing. SALL2 contains seven C2H2-type zinc-finger regions. Its highest expression is observed in different areas of adult brain and lower levels are detected in heart muscle. It is expressed throughout the retina and lens vesicle as well as the periocular mesenchyme. In fetal brain it is exclusively found in pontine nuclei and is expressed at five weeks of development, the stage at which optic fissure closure begins. Mutations in SALL2 gene are known to cause Coloboma, ocular, autosomal recessive syndrome that results in abnormal morphogenesis of the optic cup and stalk, and incomplete fusion of the fetal intra-ocular fissure during gestation.

Immunogen

GST/His-tagged recombinant fragment corresponding to 90 amino acids from the C-terminal region of human Sal-Like protein 2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in H9 human embryonic stem cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SALL2 in H9 human embryonic stem cell lysate.

Specificity

Clone 2B9.1 detects Sal-like protein 2 in human cells. It targets an epitope within 90 amino acids from the C-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~130 kDa observed; 105.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).