Anti-NANOG; clone 7F7.1

Analysis Note

ControlH9 human embryonic stem cell lysate

Application

Research Sub CategoryTranscription Factors

Anti-NANOG Antibody, clone 7F7.1 is an antibody against NANOG for use in Western Blotting, ICC.

Research CategoryStem Cell Research

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NANOG in H9 human embryonic stem cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NANOG (Homeobox protein NANOG) is a member of the Nanog homeobox family of DNA-binding proteins. It is expressed in embryonic stem cells and confers pluripotency on these cells. Once embryonic stem cells become differentiated, NANOG expression is suppressed. NANOG is involved in the Hedgehog/Gli1 signaling pathway which has been implicated in the development and growth of various types of tumors.

Immunogen

GST-tagged recombinant protein corresponding to human NANOG.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in H9 human embryonic stem cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NANOG on 10 µg of H9 human embryonic stem cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~39 kDa observed