Anti-S100A4; clone 9D5.1

Application

Anti-S100A4 Antibody, clone 9D5.1 is an antibody against S100A4 for use in Western Blotting, Immunocytochemistry.

Research CategoryApoptosis & Cancer

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected S100A4 in HUVECs, HeLa, A431, and NIH/3T3 cells.

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein S100-A4 (UniProt P26447; also known as Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4) is encoded by the S100A4 (also known as CAPL, MTS1, P9KA, PEL98) gene (Gene ID 6275) in human. S100A4 belongs to a family of low moleclar weight proteins characterized by two calcium-binding helix-loop-helix EF-hand domains (a.a. 12-47 & 50-85 of human S100A4). S100 proteins exist as non-covalent homodimers with cell-specific expression patterns. S100A4 associates with cytoskeletal proteins, such as myosin and F-actin, as well as other actin-binding proteins such as Talin and integrins, and is involved in controlling not just shape and motility of normal cells, but also plays a role in cancer cells invasion/metastasis.

Immunogen

GST-tagged recombinant human S100A4 internal fragment.

Epitope: Within EF-hands 1&2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected S100A4 in 10 µg of HeLa cell lysate.

Specificity

Clone 9D5.1 was raised against human S100-A4 internal fragment that shares high sequence homology with the corresponding region in mouse (96%) and rat (93%) protein.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~12 kDa oberved. 11.73/11.72/11.78 kDa (human/mouse/rat) calculated. Uncharacterized band(s) may appear in some lysates.