Application

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Vasohibin 2 in human placenta tissue.
Immunohistochemistry Analysis: A representative lot detected Vasohibin 2 in human and mouse embryonic lung tissue (Shibuya, T., et al. (2006). American Heart Association. 26:1051-1057).

Anti-Vasohibin-2 antibody, clone 5E3 is an antibody against Vasohibin-2 for use in western blotting & IHC.

General description

Vasohibin-2, also known as Vasohibin-like protein, and encoded by the gene VASH2/VASHL, is a developmental regulator protein of angiogenesis induced by VEGF and present in various embryonic organs at six to 12 twelve weeks of development in humans. Vasohibin-2 acts as an angiogenesis inhibitor and is thought to help regulate organ vessel formation and maturation. In cancer, Vascohibin-2 appears to act to promote angiogenesis and is expressed in many cancers. In cancer cells, there are two isoforms. VASH2 (with 355 amino acid residues) is located in the cytoplasm while VASH2 (with 311 amino acid residues) is located in the nucleus. The former was found to be a relatively low abundance protein.

Immunogen

KLH-conjugated linear peptide corresponding to human Vasohibin 2.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives .

Format: Purified

Quality

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Vasohibin 2 in 10 µg of HUVEC cell lysate.

Target description

~40 kDa observed. Uncharacterized band(s) may appear in some lysates.