Anti-Atg13; clone 2H4.2

Analysis Note

ControlCampthothecin treated HeLa cell lysate.

Application

Western Blot Analysis: A representative lot detected Atg13 in HeLa cell lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Atg13 in normal human substancia nigra tissues.

Research CategoryApoptosis & Cancer

This Anti-Atg13 Antibody, clone 2H4.2 is validated for use in Western Blotting, IHC for the detection of Atg13.

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The autophagy-related protein 13 (Atg13) plays an important role in the formation of autophagosomes. Autophagosomes are formed in response to nutrient deprivation and function as the transport vesicles for organelles, proteins, and protein complexes targeted for lysosomes that digest these cargos to produce energy and nutrients. Atg13 is activated by the mTOR pathway and forms a complex with the FIP200 protein. This complex is involved in enhancing the activity of the ULK1 kinase which is required for the formation of autophagosomes. Atg13/FIP200 faciliates the localization of ULK1 to pre-autophagosomes, and subsequently stabilizes ULK1. Autophagy is an important process in development, growth, and cell differentiation, and disruption of this process may contribute to cancer, aging, and neurodegenerative diseases.

Immunogen

GST-tagged recombinant protein corresponding to human Atg13.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in campthothecin treated HeLa cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Atg13 in 10 µg of campthothecin treated HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~60 kDa observed