Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

This Anti-phospho-ULK1 (Ser 467) antibody, clone 15D12.3 is validated for use in western blotting for the detection of phospho-ULK1 (Ser 467).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.

Immunogen

KLH-conjugated linear peptide corresponding to human phospho-ULK1 (Ser 467).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgMλ supernatant in buffer containing PBS with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in A-431 serum starved cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected phospho-ULK1 (Ser467) in 10 µg of A-431 serum starved cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~112 kDa observed