Analysis Note

ControlMBP tagged recombinant Isocitrate Dehydrogenase 1 (IDH1)

Application

Research CategoryApoptosis & Cancer

Western Blot Analysis: A representative lot of this antibody detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in a panel of wild-type and mutated recombinant IDH1 proteins.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in anaplastic and diffuse astrocytoma tissues and in primary glioblastoma tissues (Kato, Y., et al. (2009). Biochem Biophys Res Commun. 390(3):547-51.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Isocitrate Dehydrogenase 1 (IDH1)-R132S in transfected glioblastoma and transfected LN229 cells (Kaneko, M. K., et al. (2011). Biochem Biophys Res Commun. 406(4):608-613.).

Research Sub CategoryApoptosis - Additional

Detect Isocitrate Dehydrogenase 1 (IDH1)-R132H using this Anti-Isocitrate Dehydrogenase 1 (IDH1)-R132H Antibody, clone HMab-1 validated for use in Western Blotting, IHC, ICC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Isocitrate dehydrogenase 1 (IDH1) is a 46 kDa NADP-dependent enzyme, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate. IDH1 may also play a role in the prevention of oxidative damage, and the shuttling of proteins to peroxisomes. It is widely reported that mutations in IDH1 result in various types of glioma.

Immunogen

KLH-conjugated linear peptide corresponding to human Isocitrate Dehydrogenase 1 (IDH1).

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in MBP-tagged recombinant Isocitrate Dehydrogenase 1 (IDH1).

Western Blot Analysis: A 1:500 dilution of this antibody detected MBP tagged recombinant Isocitrate Dehydrogenase 1 (IDH1) in WB.

Specificity

This antibody is specific for the R132H mutation of Isocitrate Dehydrogenase 1 (IDH1).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~90 kDa observed. The calculated molecular weight of Isocitrate Dehydrogenase 1 (IDH1) is 47 kDa However, a Western Blot was performed using an MBP-tagged recombinant Isocitrate Dehydrogenase 1 (IDH1). Because MBP has a molecular weight of ~42 kDa, a band was observed at ~90 kDa