Anti-FIP200; clone 14E11.2

Analysis Note

ControlHeLa cell lysate

Application

Research CategoryApoptosis & Cancer

Western Blot Analysis: 1 µg/mL from a representative lot detected FIP200 in serum starved and non-serum starved HeLa cell lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FIP200 in NIH/3T3, A431, and HeLa cells.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected FIP200 in untreated and chloroquinone-treated HUVEC cells.

Research Sub CategoryApoptosis - Additional

Anti-FIP200 Antibody, clone 14E11.2 is an antibody against FIP200 for use in Western Blotting, ICC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Focal adhesion kinase family interacting protein of 200 kDa (FIP200) is a novel protein inhibitor for focal adhesion kinase (FAK). FIP200 inhibits kinase activity and associated cellular functions, such as cell adhesion, spreading, and motility in fibroblasts by binding directly to FAK.

Immunogen

GST-tagged recombinant protein corresponding to the interaction domain of human FIP200.

Epitope: Interaction domain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected FIP200 in 10 µg of HeLa cell lysate.

Specificity

This antibody binds to the interaction domain of FIP200, which is needed for the formation of the ULK-FIP200 complex under starved, autophagy conditions.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~200 kDa observed. An uncharacterized band may be observed at ~50 kDa in some cell lysates.