Anti-MTH1; clone 11A3.2

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Anti-MTH1 Antibody, clone 11A3.2 is an antibody against MTH1 for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

7,8-dihydro-8-oxoguanine triphosphatase (EC 3.6.1.55; UniProt P36639; also known as 2-hydroxy-dATP diphosphatase, 8-oxo-dGTPase, Nucleoside diphosphate-linked moiety X motif 1, Nudix motif 1) is encoded by the NUDT1 (also known as MTH1) gene (Gene ID 4521) in human. Human MTH1/NUDT1 is the bacterial mutT ortholog that functions as a nucleotide pool-sanitising enzyme. MTH1/NUDT1 cleaves oxidized nucleotides such as 8-oxo-deoxyguanosinetri-phosphate (8-oxo-dGTP) and converts the triphosphates into the corresponding mono-phosphates. The hydrolysis reaction assures that the oxidized nucleotides can no longer be recognized by DNA polymerase, thereby preventing bases mispairing and transversion mutations by avoiding their incorporation during DNA replication. Upregulated MTH1 levels are found in cancer cells as a means to reduce 8-oxo-dGTP accumulation in the nucleotide pool due to high oxidative stress levels in these cells.

Immunogen

GST-tagged recombinant human MTH1 C-terminal fragment.

Epitope: C-terminal half.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MTH1 in 10 µg of Jurkat cell lysate.

Specificity

Clone 11A3.2 recognizes an epitope present in all four spliced isoforms of human NUDT1/MTH1 reported by UniProt (P36639).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~20 kDa observed. 22.52 kDa (isoform p26), 20.30 kDa (isoform p22), 19.47 kDa (isoform p21), 17.95 kDa (isoform p18) calculated. Uncharacterized band(s) may appear in some lysates.