Application

Research Sub CategoryIsotype Control Antibodies

Phycoerythrin conjugated mouse IgG1 is a monoclonal antibody intended for use as a negative control in flow cytometry. Its use enables an estimation of non-specific binding of phycoerythrin conjugated mouse monoclonal antibodies to cell surface components in peripheral blood.

SUGGESTED USAGE

Flow cytometry - use 10 µl direct from the vial per 100 µl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 µl buffer.

Research CategorySecondary & Control AntibodiesEpitope Tags & General Use

Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Chicken immunoglobulin

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified immunoglobulin. R-Phycoerythrin* conjugatedready to use. The antibody is supplied in 1.0ml phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.

Specificity

This antibody reacts with chicken immunoglobulin. No reaction with human cell surface or plasma components has been observed. However some binding to Fc receptors may occur.

Storage and Stability

Store at 2 to 8°C. DO NOT FREEZE. Protect from light, for up to 6 months.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.