Application
Anti-Prion Protein Antibody is an antibody against Prion Protein for use in ELISA, WB, IH.
Western blot: 1:50,000-1:200,000
Immunohistochemistry: 1:100-1:500. Epitope must be re-exposed in fixed tissue by pretreatment of tissue using one of the following procedures:
a. formic acid for 10 minutes at room temperature
b. hydrolytic autoclaving
c. heat induced epitope retrieval
ELISA: 1:20,000-1:50,000
Optimal working dilutions must be determined by end user.
Research Sub CategoryNeurodegenerative Diseases
Research CategoryNeuroscience
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Recombinant PrP amino acids 23-237.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in PBS. Contains no preservative.
Specificity
Reacts with prion protein (PrP) from bovine, sheep, mule deer, elk and mouse. The monoclonal recognizes both protease sensitive and protease resistant forms of PrP (after denaturing).
Storage and Stability
Maintain at -20°C to -70°C in undiluted aliquots for up to 6 months after date of receipt.
Avoid repeated freeze/thaw cycles.
This product has met the following criteria to qualify for the following awards: