Analysis Note

ControlPositive Control: Brain (sympathetic nerve terminals) and adrenal gland tissue

Application

This Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15 is validated for use in IH, WB for the detection of Tyrosine Hydroxylase.

Research CategoryNeuroscience

Immunohistochemistry:
1:100-1:500. A previous lot of this antibody was used in immunohistochemistry.

Western blot:
1:1000-1:2000

Optimal working dilutions must be determined by end user.

Research Sub CategoryNeurotransmitters & ReceptorsNeuronal & Glial Markers

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Immunogen

Purified tyrosine hydroxylase (EC 1.14.16.2) from a rat pheochromocytoma (2).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Format: Purified

Purified mouse monoclonal IgG2a in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.

Quality

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 µg of PC12 lysates.

Specificity

Tyrosine hydroxylase (TH) catalyzes the rate-determining initial step in the biosynthesis of catecholamines such as dopamine, noradrenaline and adrenaline. Therefore the antibody is used to stain dopaminergeous and adrenergeous neurons as well as the chromaffine tissue of the adrenal medulla. Cell lines such as the PC 12 line, which is derived from a rat pheochromocytoma also, show positive reactions. In immunoblots of adrenal medulla tissue, the antibody recognizes a single protein band in the molecular weight range of 56 - 60 kD.

Storage and Stability

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

Target description

56-60 kDa