Analysis Note

ControlRat oligodendrocyte cells

Application

Detect Galactocerebroside using this Anti-Galactocerebroside Antibody, clone mGalC, Alexa Fluor^®488 Conjugate validated for use in ICC.

General description

Galactocerebroside (GalC) is a major galactosphingolipid of myelin which plays a role in myelination. GalC is a very useful, specific marker for oligodendroglial lineage.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Physical form

Purified mouse monoclonal IgG3 conjugated to Alexa Fluor^® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Quality

Evaluated by Immunocytochemistry in rat oligodendrocyte cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Galactocerebroside in rat oligodendrocyte cells. Performed without Triton.

Specificity

This antibody recognizes Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold to a lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically with oligodendrocytes and Schwann cells.