CYTOKERATIN 8; MSX-50UG (R)

Application

This Anti-Cytokeratin 8 Antibody, clone 4.1.18 is validated for use in WB, IC, IH, IH(P) for the detection of Cytokeratin 8.

Western blot Immunocytochemistry Immunohistochemistry: Frozen sections, acetone fixed (5-10 µg/mL)

Paraffin sections, after trypsin treatment (5-20 µg/mL)

Optimal-working dilutions must be determined by end user.

Immunogen

Cytoskeletal preparation of MCF-7 cells.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-588

Physical form

Lyophilized from 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.

Format: Purified

Specificity

The antibody reacts with human cytokeratin 8. There is no cross reactivity with human cytokeratin No. 7, 17, 18, or 19. Anti-cytokeratin No. 8 decorates single epithelia in stomach, colon, and small intestine, gall bladder, liver (bile ducts and hepatocytes) as well as pancreatic ducts and mammary gland ducts.