Analysis Note

ControlHeLa Cell lysateA431 Cell lysateMCF7 Cell lysate

Application

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

This Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin.

Immunocytochemistry:
A 1-2 µg/mL concentration of a previous lot was used in immunocytochemistry.

Western blot:
0.05-0.5 µg/mL

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.

Immunogen

Tubulin purified from polycephalum myxamoebae

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1049

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Format: Purified

Purified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Quality

Routinely evaluated by Western Blot on A431 lysates.

Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 µg of A431 lysates.

Specificity

This antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only.

Storage and Stability

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

Target description

50 kDa