MMP-3; MSX-100UG

Analysis Note

ControlPOSTIVE CONTROL:

Human endometrium cells, placenta, bladder, breast, ovarian carcinomas.

Application

Immunofluorescence

Immunoprecipitation (Use Protein A): 2µg/ml protein lysate.

Western blotting: 0.5-1.0 µg/ml for 2hrs at RT

Immunohistology (frozen & formalin-fixed): 10-20 µg/ml for 60 minute at room temperature. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissue sections.

Optimal working dilutions and protocols must be determined by end user.

Research CategoryCell Structure

Research Sub CategoryMMPs & TIMPs

Anti-MMP-3 Antibody, clone SL-1 IID4 is an antibody against MMP-3 for use in ELISA, IP, WB, IH(P).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

MMPs are frequently expressed in malignant neoplastic cells and play crucial roles in tumor invasion and metastasis. Tissue inhibitors of matrix metalloproteinases (TIMPs) control the activitiy of MMPS. The tissue distribution of three major MMPs has been defined in human mammary pathology: 1) collagenase (MMP-1) which degrades fibrillar interstitial collagens; 2) a 72-k/Da gelatinase (MMP-2) which mainly degrades type IV collagen and denatured collagens; and 3) stromelysin (MMP-3) which has a wider range of action, degrading several matrix components including the core proteins of proteoglycans, laminin and non-helical regions of collagens.

Immunogen

APMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified from ascites fluid by Protein A chromatography. 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Format: Purified

Specificity

MAB3369 recognizes one doublet of 54kDa/59kDa and another doublet of 44kDa/49kDa, which are identified as unglycosylated and glycosylated species of pro (latent) and active forms of matrix metalloproteinase-3 (MMP-3; also known as Stromelysin-1 of Transin). MAB3369 shows no cross-reaction with the pro and active forms of other MMPs.

CELLULAR LOCALIZATION:

Cytoplasmic

Storage and Stability

Antibody with sodium azide is stable for 12 months when stored at 2-8°C.