Application
Anti-Cytokeratin 18 Antibody, clone RCK106 detects level of Cytokeratin 18 & has been published & validated for use in FC, WB, IC, IH(P).
Western blot
Immunohistochemistry on frozen and paraffin embedded tissue sections.
Immunocytochemistry
Flow cytometry
Optimal working dilutions must be determined by the end user.
Research CategoryCell Structure
Research Sub CategoryCytokeratins
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
Immunogen
Cytoskeletal preparation of human bladder carcinoma cell line T24.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Linkage
Replaces: 04-586
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Liquid in buffer with 0.1% sodium azide.
Format: Purified
Specificity
Cytokeratin 18. MAB3236 reacts exclusively with human glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells as well as adenocarcinomas originating from them.
SPECIES REACTIVITY:
Human only. Does not react with other species.
Storage and Stability
Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
This product has met the following criteria to qualify for the following awards: