DYKDDDK; MS X -100UL

Analysis Note

ControlCells transfected with FLAG-tagged fusion vector

Application

Detect DYKDDDDK (FLAGepitope tag) using this Anti-DYKDDDDK (FLAG epitope tag) Antibody, clone 2EL-1B11 validated for use in ELISA, IC, IF, IP & WB.

Research Sub CategoryEpitope Tags

Immunofluorescence:
A 1:1,000-1:5,000 dilution of a previous lot was used in IF.

Immunoprecipitation:
A 1:1,000-1:5,000 dilution of a previous lot was used IP on recombinant proteins.

ELISA:
A 1:1,000-1:5,000 dilution of a previous lot was used in Enzyme-linked immunosorbent assay on recombinant proteins.
Note: Does not require Ca++ for binding.

Immunoblotting:
on recombinant proteins

Optimal working dilutions must be determined by the end user.

Research CategoryEpitope Tags & General Use

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

FLAG^® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Immunogen

Epitope: Epitope tag

Synthetic peptide M-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys(C) coupled to ovalbumin

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Linkage

Replaces: AB3250

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Ascites mouse monoclonal IgG1 liquid containing no preservative.

Unpurified

Quality

Routinely evaluated by Western Blot on Flag-mTor protein blot.

Western Blot Analysis:
1:500 dilution of this lot detected Flag mTor on Flag-mTOR blot.

Specificity

DYKDDDDK sequence containing proteins. Reacts with both N-terminal and C-terminal fusion proteins.

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.