AntiConnexin45NearCT;cytoplasmic;Clone8A

Application

Immunocytochemistry Analysis: A representative lot detected Connexin 45 in transfected rat C6 cells.

Immunocytochemistry Analysis: A representative lot detected Connexin 45 in Rat aorta endothelial cells and transfected HeLa cells (Sorensen, C.M., et al. (2008) Am J Physiol Regul Integr Comp Physiol. 295:R840–R847).

Immunofluorescence Analysis: A representative lot detected Connexin 45 in HeLa cells and human cardiomyocytes (Hagen, A., et al. (2009) Cardiovascular Research. 83:405–415).

Immunofluorescence Analysis: A representative lot detected Connexin 45 in normal adult rat bladder (Ikeda Y., et al. (2007) Am J Physiol Renal Physiol. 293(4):F1018–F1025).

Western Blotting Analysis: A representative lot detected Connexin 45 in HeLa and human cardiomyocyte lysate (Hagen, A., et al. (2009) Cardiovascular Research. 83:405–415).

Western Blotting Analysis: A representative lot detected Connexin 45 in guinea pig atrial and SA node tissue (Jones S.A., et al. (2004) The Journal of Physiology, 560:429-437).

Western Blotting Analysis: A representative lot detected Connexin 45 in WT and transfected HeLa cell lysates (Elenes S., et al. (2001) Biophys J. 81(3):1406-18).

Immunohistochemistry Analysis: A representative lot detected Connexin 45 in neural tube of 12-day-old embryo and bone marrow spaces and blood vessels after 4 days of orthodontic treatment in periodontium (Gluhak-Heinrich, J., et al. (2006) Cell Commun Adhes. 13(1-2):115–125).

Immunohistochemistry Analysis: A representative lot detected Connexin 45 in endothelial and subintimal smooth muscle gap junction plaques in the rabbit iliac artery (Chaytor, A.T., et al. (2005) British Journal of Pharmacology. 144:108–114).

Immunohistochemistry Analysis: A representative lot detected Connexin 45 in rabbit middle cerebral artery (Ujiie H., et al. (2003) Stroke. 34(2):544-50).

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

This Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2 (Ascites Free) is validated for use in Western Blotting and Immunocytochemistry and Immunofluorescence and Immunohistochemistry for the detection of Connexin 45, near CT, cytoplasmic.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Connexin 45 is member of a family of gap junction proteins known as Connexin gene family. It is expressed in a wide variety of cell types during development and adult life, particularly in cardiac and smooth muscle tissues as well as brain tissues. Connexin 45 forms part of a network of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. There a number of alternatively spliced transcript variants that all seen to encode the same protein isoform.

Immunogen

KLH-conjugated linear peptide corresponding to Human Connexin 45.

Linkage

Replaces: MABC3100

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Connexin 45 in 10 µg of U2OS cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~45 kDa observed.
Additional uncharacterized bands may be visible in some lysates.