Application

Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2 detects level of Dopamine β Hydroxylase & has been published & validated for use in IH, IH(P) & IF.

Indirect immunofluorescence:
1:300-1:500 dilution of a previous lot was used on rat brain tissue.

Optimal working dilutions must be determined by the end user.

Immunohistochemistry(paraffin):
Representative images from a previous lot.

Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord

General description

Dopamine Beta Hydroxylase (DBH), the structural locus encoding DβH enzyme, is a major quantitative trait locus influencing plasma DβH activity, with one single nucleotide polymorphism (SNP) accounting for up to 50% of the variance. Mutations at DBH appear to be responsible for the rare syndrome of DβH deficiency. It functions as the only enzyme involved in the synthesis of small-molecule neurotransmitters that is membrane-bound, making norepinephrine and epinephrine the only transmitters synthesized inside vesicles. It is expressed in noradrenergic nerve terminals of the central and peripheral nervous systems, as well as in chromaffin cells of the adrenal medulla.

Immunogen

Purified bovine DBH.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MAB394-250UG; MAB394-100UG; MAB394-25UG

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Ascites mouse monoclonal IgG1 in liquid with 0.1% sodium azide.

Quality

Immunohistochemistry(paraffin): Dopamine β Hydroxylase (cat. # MAB308) staining pattern/morphology in rat spinal cord. Tissue was pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:300, using IHC-Select™ Detection with HRP-DAB. Immunoreactivity is seen predominantly expressed in the dorsal root ganglia (DRG).

Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord

Specificity

Dopamine beta hydroxylase (DBH).