Application
Anti-Integrin β4 Antibody, clone ASC-8 is an antibody against Integrin β4 for use in FC, IP, IH.
Research Sub CategoryIntegrins
Immunoprecipitation: A representative lot of this antibody clone precipitated PAGE bands of 66, 76, 120, 135 and 200 kDa (reduced) or 66, 110, 125, 130 and 190 kDa (non-reduced) from surface-biotinylated SCC9 cells.
Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue).
Flow Cytometry: A representative lot of this antibody clone stained human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.
Functional Activity Assay: A representative lot of this antibody clone induced a sub-basal split at the basement membrane in non-wounded tissue. In addition, wound closure was significantly inhibited by ASC-8, as the epithelial tongue only covered 40 percent of the wound area at 120 hours post-wounding. These results demonstrate that the β4 adhesion-blocking antibody may have adverse effects on normal tissue (Egles, C., et al., 2010).
Working dilutions must be determined by end user.
Research CategoryCell Structure
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Human squamous cell carcinoma cells (SCC9)
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Linkage
Replaces: 05-283
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Format: Purified
Quality
Flow Cytometry Analysis:
0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 A431 cells.
0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 HeLa cells.
Specificity
Human beta4 integrin (CD104)
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
This product has met the following criteria to qualify for the following awards: