INTEGRIN B1; MS X-100UG

Application

Research Sub CategoryIntegrins

This Anti-Integrin β1 Antibody, clone P4G11, azide free is validated for use in ELISA, FC, IP, FUNC, FUNC, IC, IH for the detection of Integrin β1.

Research CategoryCell Structure

Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}

Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

Traditional formalin fixation is not recommended.

Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)

Immunoprecipitation: 5 µg/400 µL lysate (0.5% triton PBS plus Ca++)

FACS: 2-5µg per 10e6 cells, in the presence of Ca++.

Stimulation of cells: 2-10 µg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.

ELISA

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Human IL-1beta activated endothelial cells

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Sterile-filtered protein A purified immunoglobulin in 0.01M PBS, pH 7.1, containing no preservatives.

Format: Purified

Specificity

Human integrin beta1. The epitope recognized by MAB1951Z is Ca2+ dependent.

Storage and Stability

Maintain between 2 and 8°C for up to 6 months from date of receipt. Avoid freeze thaws.