Analysis Note

ControlTubular carcinoma of the colon and fibroneuroma

Application

Detect Laminin gamma 1 using this Anti-Laminin gamma 1 Antibody, clone A5 validated for use in IH(P).

Research Sub CategoryECM ProteinsAdhesion (CAMs)

General description

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Variations in the expression of laminins have been observed in embryogenesis, organogenesis, post traumatic healing and cancer. Laminins have been provoked by the discovery of their ability to promote neurite regeneration. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively). The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly.

Immunogen

Basement membrane protein preparation from mouse EHS tumor.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rat immunoglobulin in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN₃.

Format: Purified

Target description

177 kDa