COLLAGEN I; II; III; MS X-100UG

Application

ELISA: Apparent binding constant from titration curves is 0.1 nM. Can be used as capture or reporter antibody for determination of collagen I or III in sandwich ELISA, but requires combination with collagen type-specific antibody for other function.

Immunohistochemistry: cryostat sections fixed with acetone or ethanol. Staining pattern similar to polyclonal antibody to interstitial collagens. Formaldehyde fixation is not recommended.

Not suitable for use on paraffin embedded sections or immunoblots.

Optimal working dilution must be determined by the end user.

Research CategoryCell Structure

Anti-Collagen Types I, II & III Antibody, clone MMCHABC detects level of Collagen Types I & has been published & validated for use in ELISA, RIA, IH.

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Human collagen type I

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Sepharose is a registered trademark of Cytiva

Physical form

Purified from murine ascites by PEG precipitation and Sepharose^® CL-4B chromatography. Lyophilized Powder.

Format: Purified

Specificity

Human collagen types I, II and III (both native and denatured). No cross reactivity with human plasma proteins, other collagen types or non-collagenous proteins in ELISA, RIA or immunoblot.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.