Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunofluorescence (1 paper)

Monoclonal Anti-Human Gastric Mucin may be used for the localization of gastric mucin using various immunochemical assays such as immunoblot, immunofluorescence, immunohistochemistry, immunostaining and immunoradiofixation.

Biochem/physiol Actions

Mucin is composed of a peptide core containing heavily glycosylated regions and nonglycosylated regions. The heavily glycosylated components of mucin consist of a mosaic of epitopes, some of which are associated with saccharide moieties and are related to blood-group antigens, and others which are M1 antigens associated with the peptidic core. M1 antigens are common to human ovarian mucinous cyst fluids and to gastric mucosa. M1 is distributed in various human tumors such as the colon, stomach, pancreas, ovary, Barrets′ esophagus, endocervix, and endometrium carcinomas.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mucin has a netlike, branched structure and is a secreted protein. It belongs to the category of gel-forming mucins. The gene encoding this protein is upregulated by interleukin-1β (IL-1β) and is localized on chromosome 11p15.5.

Monoclonal Anti-Human Gastric Mucin (mouse IgG1 isotype) is derived from the 445M1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with mucin isolated from human ovarian cyst fluid. Mucin is a high M.W. (1,000 kDa) glycoprotein, expressed by mucus cells of the gastric epithelium and by goblet cells of the fetal, precancerous and cancerous colon, but not by those of the normal colon. It also appears in other epithelial tissues, which are embryologically derived from the foregut (epigastric and bronchial epithelium) and in Müller ducts (mucus cells of the endocervix and urethral epithelium near the prostatic utriculus).

Immunogen

mucin from human ovarian cyst fluid.

Specificity

The antibody recognizes the mucin epitope g located in the peptide core of gastric mucin (>1,000 kDa). This epitope is completely destroyed by thiol reduction (using mercaptoethanol) and partially lost following trypsin proteolysis, but is stable upon periodate oxidation. The antibody stains the surface gastric epithelium of normal human gastrointestinal tract and reacts with fetal, pre-cancerous and cancerous colonic mucosa, but not with normal colon.