Application

Monoclonal Anti-MAP1b antibody produced in mouse has been used in:immunohistochemistry immunostainingfluorescence microscopy

Biochem/physiol Actions

Microtubule-associated protein (MAP1B) is the first MAP to appear in growing axons during development as it is present from the first emergence of the nascent axon from the cell body. Monoclonal Anti-MAP1b may be used to study MAP expression and cytological localization both in tissues and cell lines, under different developmental and environmental circumstances.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-MAP1b (MAP5) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Microtubule-associated protein (MAP1B) is the major microtubule associated protein in developing brain which changes its expression during development. In the new born rat brain, it is a major component of microtubules but in the adult its level is ten-fold lower.

MAP1b is a microtubule associated protein that interacts with actin filaments. MAP1b regulates microtubule stabilization and the development of axons. It may also act as a scaffolding protein Addition of antibody to microtubule proteins before polymerization in immunoassays results in abnormally short (but otherwise morphologically normal) microtubules. Immunohistochemical staining of brain tissue with the product shows selective labeling of dendritic trees throughout the brain. Monoclonal Anti-MAP1b does not react with tubulin of other microtubule associated proteins.

Immunogen

rat brain microtubule-associated proteins (MAPs)

Specificity

Monoclonal Anti-MAP1b antibody is specific for MAP1b in humans, mice, rats, bovines, cats, hamsters and chickens. The antibody does not cross-react with other MAPs or tubulin. Addition of the antibody to microtubule proteins before polymerization results in abnormally short (but otherwise morphologically normal) microtubules. In immunohistochemical staining of brain tissue, the antibody shows selective labeling of neurons with stronger staining of axons, dendrites and cell bodies.