Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2)

Analysis Note

Positive ControlCaR-1 or TPA treated SW620 cells

Application

Immunoblotting (5-10 g/ml, see application references)Paraffin Sections (see application references)

General description

Recognizes the ~28 kDa latent form of MMP-7 in TPA-treated SW620 cells. Does not recognize the active form of MMP-7.

Purified mouse monoclonal antibody. Recognizes the ~28 kDa latent form of MMP-7.

This Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2) is validated for use in Immunoblotting, Paraffin Sections for the detection of MMP-7 (Ab-1).

Immunogen

human rectal carcinoma MMP-7

Human

Legal Information

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not recognize the ~19 kDa active form of MMP-7. To detect active MMP-7, use Cat. No. IM47L. Does not cross-react with human MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 or MMP-13. For optimal detection, SW620 cells should be treated with TPA (100 ng/ml) to induce expression of MMP-7. Antibody should be titrated for optimal results in individual systems.

Brunner, K.L, et al. 1995. Proc. Natl. Acad. Sci. USA92, 7362.Imai, K., et al. 1995. J. Biol. Chem.270, 6691.Lichtinghagen, R., et al. 1995. Eur. J. Clin. Chem. Clin. Biochem.33, 65.Nakano, A., et al. 1995. J. Neurosurg.83, 298.Takino, T., et al. 1995. J. Biol. Chem.270, 23013.Woessner, J.F. 1991. FASEB J.5, 2145.Yamamoto, H., et al. 1995. J. Clin. Lab. Anal.9, 297.Gaire, M., et al. 1994. J. Biol. Chem.269, 2032.McDonnell, S., et al. 1994. Biochem. Soc. Trans.22, 58.Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol.2, 861.Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.Woessner, J.F. 1991. FASEB J.5, 2145.Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.Woessner, J.F., Jr and Taplin, C.J. 1988. J. Biol. Chem.263, 16918.Sellers, A. and Woessner, J.F., Jr. 1980. Biochem. J.189, 521.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)