BB2 MEMBRANE PREPARATION

Application

Radioligand binding assay and GTPγS binding.

Human BB2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

Biochem/physiol Actions

Target Sub-Family: Bombesin

GPCR Class: A

Protein Target: BB2

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Full-length human GRPR cDNA encoding BB2

Bombesin, a bioactive peptide first identified in amphibian skin, is related to two mammalian peptides, gastrin-releasing peptide (GRP) and neuromedin B. A family of 3 GPCRs, including NMB-R (BB₁), GRP-R (BB₂) and BRS-3 (BB₃), mediate the biological effects of the peptides. The receptors differ in their affinities for the peptides. Binding of ligand to BB₂ activates Gq to increase intracellular calcium concentrations. GRP stimulates release of gastrin from endocrine cells and stimulates smooth muscle activity in the gastrointestinal tract. In addition, binding of GRP to BB₂ stimulates proliferation of a variety of cell types, and has been implicated in the progression of small cell lung cancer and other malignancies. The CNS is also a major site of GRP expression, and GRP and BB₂ are involved in the circadian clock, conditioned fear, and food intake (Ohki-Hamazaki et al., 2005). Chemicon′s BB₂ membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of BB₂ interactions and its ligands. The membrane preparations exhibit a Kd of 0.114 nM for [¹²⁵I]-Tyr4-Bombesin. With 0.3nM [¹²⁵I]-Tyr4-Bombesin, 5 µg/well or 10 µg/well BB₂ Membrane Prep typically yield greater than 20-fold signal-to-background ratio.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.
Packaging method: Membranes protein were adjusted to 0.5 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C.

Quality

Signal:background and specific binding values obtained in a competition binding assay with varying amounts of BB2 membrane prep:

Specifications

Inucbation ConditionsMembranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Tris, pH 7.4, 10 mM MgCl2, 1 mM EDTA, filtered and stored at 4°C
Radioligand: [¹²⁵I]-Tyr4-Bombesin (Perkin Elmer#:NEX-258 )
Wash Buffer: 50 mM Tris, pH 7.4 filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 10-fold signal:background with ¹²⁵I labeled Bombesin at 0.3 nM

Storage and Stability

Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.