Monoclonal Anti-HLA Class I Antigen antibody produced in mouse, clone W6/32, purified immunoglobulin, buffered aqueous solution

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunofluorescence (1 paper)

Biochem/physiol Actions

HLA class I molecules like HLA-A,-B and −C help in the presentation of endogenous antigen to CD8+ (cluster of differentiation 8) T cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Class I proteins are highly polymorphic, in which HLA-Bseems to be the most polymorphic gene in the human genome. HLA-A, -B, -C are classical class I proteins and are heterodimers. HLA-A, -B, -C consists of a polymorphic transmembrane α heavy chain and an invariant water-soluble light chain. HLA-B is located on human chromosome 6p21.

Immunogen

human tonsil leukocyte membrane preparation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Specificity

Recognizes the human HLA Class I antigen expressed on most human nucleated cells. The antibody reacts with a monomorphic epitope on the 44-45 kDa α-chain polypeptide of human class I HLA molecules (HLA-A,B,C). The antibody may be used in immunoprecipitation, complement mediated cytotoxicity, flow cytometry and immunohistochemical staining of acetone-fixed, frozen sections and cell smears. It cross-reacts with cells from African and Asian apes and old world monkeys. No reactivity is seen with cells from most new world monkeys or from non-primates. The epitope recognized by this antibody is sensitive to routine formalin fixation and paraffin embedding.4th Workshop: code no. P15th Workshop: code nos. BP166, BP288, BP407, B 005