ANTI-HISTONE H3

Code: H0164-25UL D2-231

Application

Anti-Histone H3 antibody produced in rabbit has been used in immunoblotting and immunofluorescence microscopy.

Disclaimer

Unless othe...


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Application

Anti-Histone H3 antibody produced in rabbit has been used in immunoblotting and immunofluorescence microscopy.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone proteins are known to stabilize and regulate the nuclear DNA. Core histones, H2A, H2B, H3 and H4 aid the packaging of eukaryotic nuclear DNA into repeating nucleosome units while the linker histone, H1, binds to DNA between the nucleosomes. A diverse and elaborate array of post-translational modifications, including acetylation, phosphorylation and methylation, ubiquitination and ADP ribosylation occur in histones, particularly on the N-terminal tail domains of histones, particularly of H3 and H4.These modifications dictate chromatin states and the gene expression. Phosphorylation of H3, mainly on Ser10 and Ser28, is localized to a small fraction of highly acetylated H3 and occurs primarily in response to mitogenic and stress stimuli. Phosphorylation of Ser10 is considered a crucial event for the onset of mitosis. It is tightly correlated with chromosome condensation during both mitosis and meiosis. Histone methylation, like acetylation, is a complex, dynamic process involved in a number of processes, including transcriptional regulation, chromatin condensation, mitosis ad heterochromatin assembly. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9, and Lys27 are the preferred sites of methylation. Histone lysine residues can be mono-, di-, and tri-methylated at different heterochromatic subdomains, adding further complexity to the regulation of chromatin structure.

Immunogen

synthetic peptide corresponding to amino acids 125-136 located at the C-terminus of human histone H3, conjugated to KLH. This sequence is identical in many species including rat, mouse, chicken, Xenopus, Drosophila, and plant histone H3.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-Histone H3 specifically recognizes histone H3.

antibody formIgG fraction of antiserum
antibody product typeprimary antibodies
biological sourcerabbit
clonepolyclonal
conjugateunconjugated
enhanced validationindependentLearn more about Antibody Enhanced Validation
formbuffered aqueous solution
Gene Informationhuman ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)rat ... H3f3b(117056)
mol wtantigen 17 kDa
packagingantibody small pack of 25 µL
Quality Level200
shipped indry ice
species reactivitymouse, chicken, Xenopus, human, rat, Drosophila, plant
storage temp.−20°C
technique(s)indirect immunofluorescence: 1:250-1:500 using mouse fibroblast NIH3T3 cells, western blot: 1:2,500-1:5,000 using whole cell extract of the rat pheochromocytoma PC12 cell line, western blot: 1:5,000-1:10,000 using whole cell extract of the A431 human epidermoid carcinoma cell line or a whole cell extract of the mouse fibroblast NIH3T3 cell line
UniProt accession no.P68431
This product has met the following criteria to qualify for the following awards:



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