Analysis Note

Positive ControlA-431 cells

Negative ControlHL-60 cells

Application

Flow Cytometry (1-5 g/ml)Immunofluorescence (1-5 g/ml)Immunoprecipitation (1-2 g/sample)Neutralization Studies (1-2 g/ml; use Cat. No. GR15L; see application references)

General description

Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, and IP.

Recognizes the ~170 kDa EGF receptor protein in A431 cells. For neutralization studies Cat. No. GR15L is recommended.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.

Immunogen

partially purified, human EGFR from A431 cells

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

EGF-Receptor (Ab-5) binds to A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.

Hunter T., 1984. Nature311, 414.Reynolds, F.H., Jr., et al. 1981. Nature292, 259.Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate, 0.2% gelatin, pH 7.5.

Warning

Toxicity: Standard Handling (A)