Analysis Note

Negative ControlHL-60 cells or human brain

Positive ControlA-431 or normal skin tissue

Application

Flow Cytometry (5 µg/ml) r>Frozen Sections (2.5 µg/ml) r>Immunofluorescence (5 µg/ml) r>Immunoprecipitation(1 µg/sample)r>Neutralization Studies (see comments and application references)r>Paraffin Sections (see application references)

General description

Recognizes the ~170 kDa EGF receptor protein in A431 cells and normal skin.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references. Recognizes the ~170 kDa EGF receptor.

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hunter T. 1984. Nature311, 414.Masui, H., et al. 1984. Cancer Res.44, 1002.Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity (see application references). Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Glass bottle

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)