Application

Goat polyclonal anti-mouse IgG (whole molecule)–Gold antibody may be used as a primary or secondary antibody that enables the labeling of mouse IgG for detection by electron microscopy.

Anti-Mouse IgG (whole molecule)-Gold antibody is suitable for use in inductively coupled plasma mass spectrometry (ICPMS)-Linked DNA Assay ((1:100). The product can also be used for immunoelectron microscopy (1:20).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Binding activity is determined using a modification of the dot blot assay of Brada and Roth. In this assay a 1 mg/mL solution of mouse IgG is serially diluted in phosphate buffered saline, 1 mL of each dilution is then applied to nitrocellulose paper. Binding activity is defined as the minimum amount of mouse IgG detectable as a visible pink-red spot after a 1 hour incubation with the gold conjugate diluted to an A520 of0.25. Relative order of singlets, doublets, triplets or greater: Singlets>Doublets>Triplets. Clustering data determined by floating the electron microscopy (EM) nickel grid on conjugate diluted 1:100 in Tris buffer, pH 8.0, containing 1% BSA, for 30-60 minutes. The grid is evaluated by EM after drying. One hundred particles are scanned to determine clustering.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (whole molecule)-Gold antibody is specific for mouse IgG.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Immunogold labeling is a technique whereing antibodies, especially secondary antibodies, are labeled with colloidal gold. These gold conjugated antibodies generate electron dense particles that are visable using electron microscopy.

Immunogen

Mouse IgG purified from normal mouse serum

Packaging

0.4 mL in glass insert

2 mL in glass bottle

Physical form

Colloidal suspension in Tris buffered saline, pH 8.2, with 30% glycerol (v/v), 1% bovine serum albumin (w/v), and 15 mM sodium azide.