Anti-Ubiquitin Alexa Flour 647; Lys63-Sp

Application

This Milli-Mark Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor™647, clone Apu3 is validated for use in FC for the detection of Ubiquitin.

General description

Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.

Immunogen

Immunogen used was di-ubiquitin linked at Lys63.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal IgG1 conjugated to Alexa Fluor 647 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Quality

Evaluated by flow cytometry using HeLa cells

Specificity

Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.

Target description

Molecular weight varies.